| Title | Salmonella typhimurium에서 MudJ(Km.lac) 오페론 융합을 이용한 삼투유도유전자의 발현 | ||
| Author | 주성관 · 우영대1 · 허연주 · 안정선2 · 박용근 | ||
| Address | 고려대학교 이과대학 생물학과; 1한남대학교 이과대학 생물학과; 2서울대학교 자연과학대학 생물학과 | ||
| Bibliography | Korean Journal of Microbiology, 29(4),215-220, 1991 | ||
| DOI | |||
| Key Words | MudJ operon fusion, osmotic-inducible genetic loci, Salmonella typhimurium, osmoprotectants, linkage mapping | ||
| Abstract | MudJ(Km.lac) operon fusions were used in the identification of osmotic-inducible genetic(osi) loci in Salmonella typhimurium. Expression of osi::lacZ(osi5001, 5027) genes were dramatically induced 39-189 fold when the osmolarity was increased. Seven osm::lacZ genes were constituvely expressed under both low and high osmotic strength. The osi5001::lacZ fusion strains showed the enhanced osmotolerance and the reduced expression of the osi5001::lacZ in the presence of 1mM proline or betaine as osmoprotectants. Four osmotic inducible genetic loci were mapped into 36 (YK531), 44 (YK504), 57 (YK501) and 84 (YK528) map unit by testing the cotransduction frequency. | ||
| Download PDF | Kor_290403_215-220p.pdf | ||