| Title | Bluetongue virus core에 의해 생산된 RNA 전사체 분석 | ||
| Author | 양재명 · JaRue S. Manning * | ||
| Address | 성균관대학교 유전공학과; * 캘리포니아대학교 대이비스 | ||
| Bibliography | Korean Journal of Microbiology, 29(4),221-225, 1991 | ||
| DOI | |||
| Key Words | Bluetongue virus, transcriptase,abortive transcription, agarose-urea gel | ||
| Abstract | The RNA transcripts produced from in vitro transcription reaction of BTV core were analyzed on agarose-urea gel. Fast migrating abortive RNAs, in addition to full length species of RNA, were observed. Fast migrating RNAs extracted from agarose-urea gel were hybridized to all 10 segments of genomic ds RNA, while solw migrating RNAs extracted from agarose-urea gel were hybridized only to the large and medium size genomic ds RNA. These results indicate that fast migrating RNA transcripts are most likely the products of abortive transcription. | ||
| Download PDF | Kor_290404_221-225p.pdf | ||