| Title | Rhizopus nigricans의 progesterone전환 반응 산물에 관한 포도당의 효과 | ||
| Author | 김명희 · 김종혜 · 김말남 | ||
| Address | 상명여자대학교 자연과학대학 생물학과 | ||
| Bibliography | Korean Journal of Microbiology, 29(4),258-262, 1991 | ||
| DOI | |||
| Key Words | Rhizopus nigricansm progesterone, 11α-hydrozylation, 5α-reduction, glucose | ||
| Abstract | Rate of 11α-hydroxylation of progesterone with Rhizopus nigricans was accelerated by glucose. Glucose seemed to play an important role in the formation of cofactor because its effects on the reaction were almost same as those of electron acceptors such as NADPH and NaIO_4. Rate of glucose consumption appeared to increase in proportion as the rate of hydroxylation reaction, which enhanced with increase in the glucose concentration to level off at 0.5 g/l for mycelia and at 20 g/l for spores. However, for mycelia immobilized in polyacrylamide gel, externally added glucose did not affect the reaction rate at all because of the glucose accumulated in the gel during the cultivation period. 5α-Reduction of 11α-hydroxyprogesternoe required much higher concentration of glucose than 11α-hydroxylation of progesternoe so that high yield of 11α-hydroxyprogesternoe can be obtained by repressing the activity of 5α-reductase at low concentration of glucose. | ||
| Download PDF | Kor_290410_258-262p.pdf | ||