Title Coprinus congregatus의 세포막 연관 laccase의 세포외 분비
Author 김순자 · 최형태 · 강사욱 * · 하영칠 *
Address 강원대학교 미생물학과; * 서울대학교 미생물학과
Bibliography Korean Journal of Microbiology, 29(5),267-269, 1991
DOI
Key Words Coprinus congregatus, enzyme secretion, liquid culture
Abstract The hyphal tip laccase of Coprinus congregatus which is a membrane-associated enzyme and shows different banding patterns of PAGE analysis when compared with the enzyme of liquid culture (Choi et al. 1987) has been successfully secreted to culture medium in liquid shake culture by lowering the pH of medium to 4.0. When the fungus is cultivated in YpSs(pH 4.0) liquid, only the hyphal tip laccase is found in the medium after 6 hr incubation and there is no liquid-type enzyme when examined by PAGE analysis.
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