| Title | 대장균으로부터 두 종류의 수소발생 동위효소의 확인과 부분정제 | ||
| Author | 최석정 · 양철학 * | ||
| Address | 강릉대학교 화학과; * 서울대학교 화학과 | ||
| Bibliography | Korean Journal of Microbiology, 29(5),296-300, 1991 | ||
| DOI | |||
| Key Words | Escherichia coli, hydrogenase isoenzymes, sodium deoxycholate, Triton X-100 | ||
| Abstract | The membrane-bound Escherichia coli hydrogenases were purified partially by the solubilization with detergents. the E. coli crude extract was solubilized with sodium deoxycholate and dialyzed against the buffer containing Triton X-100. Two different hydrogenases were obtained by the DEAE-cellulose, hydroxyapatite and Sephedex G-200 column chromatography. The one was unstable during purification and contained 70- and 47-kDa polypeptides as major proteins. The other showed high H2-evolving activity and had major polypeptides of Mr 31 and 27. Those polypeptides were detected by the two-dimensional electrophoresis. | ||
| Download PDF | Kor_290506_296-300p.pdf | ||