| Abstract |
Three types of soluble cytochrome c were purified to homogeneity from Methylobacillus sp. strain SK1 which grows only on methanol. Cytochrome c-I was purified 58.5-fold in seven steps. Cytochrome c-II and c-III were purified 57.3- and 122.1-fold in eight steps, respectively. The molecular weights of the cytochrome c-I was determined to be 12,500, while those of the cytochrome c-II and c-III were 16,000. The isoelectric points of the c-I, c-II and c-III were found to be 8.8, 6.6, and 6.6 respectively. The spectrum of reduced cytochrome c-I showed α-, β-, γ-peaks at 551.4, 522.2, and 416.6nm. The peaks for c-II were found at 551.0, 521.6, and 416.5nm, while those for c-III were shown at 551.2, 521.8, and 416.0 nm. The spectra of oxidized cytochrome c-I, c-II, and c-III showed γ-peak at 411.8, 409.0, and 410.2 nm, respectively. The absorption coefficients of α- and γ-peak for c-I in the reduced state were determined as 47 and 197 mM^-1 cm^-1, respectively. The coefficients of α- and γ- peak for c-II were determined to be 43 and 137 mM^-1 cm^-1, while those for c-III were 41 and 172 mM^-1 cm^-1, respectively. The c-I and c-III were found to bind carbon monoxide. |
