Title Methylobacillus sp. strain SK1에 존재하는 soluble cytochrome c의 정제 및 특성
Author 김시욱 · 노영태1 · 김영민1 *
Address 조선대학교 자연과학대학 환경학과; 1연세대학교 이과대학 생물학과
Bibliography Korean Journal of Microbiology, 29(6),380-386, 1991
Key Words Cytochrome c, Methylotroph, Methylobacillus sp. SK1, methanol, Methanolotroph
Abstract Three types of soluble cytochrome c were purified to homogeneity from Methylobacillus sp. strain SK1 which grows only on methanol. Cytochrome c-I was purified 58.5-fold in seven steps. Cytochrome c-II and c-III were purified 57.3- and 122.1-fold in eight steps, respectively. The molecular weights of the cytochrome c-I was determined to be 12,500, while those of the cytochrome c-II and c-III were 16,000. The isoelectric points of the c-I, c-II and c-III were found to be 8.8, 6.6, and 6.6 respectively. The spectrum of reduced cytochrome c-I showed α-, β-, γ-peaks at 551.4, 522.2, and 416.6nm. The peaks for c-II were found at 551.0, 521.6, and 416.5nm, while those for c-III were shown at 551.2, 521.8, and 416.0 nm. The spectra of oxidized cytochrome c-I, c-II, and c-III showed γ-peak at 411.8, 409.0, and 410.2 nm, respectively. The absorption coefficients of α- and γ-peak for c-I in the reduced state were determined as 47 and 197 mM^-1 cm^-1, respectively. The coefficients of α- and γ- peak for c-II were determined to be 43 and 137 mM^-1 cm^-1, while those for c-III were 41 and 172 mM^-1 cm^-1, respectively. The c-I and c-III were found to bind carbon monoxide.
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