Title Methylobacterium extorquens AM1의 메탄올을 이용한 성장과 세포내 폴리아민 구성에 미치는 배양조건의 영향
Author 엄치용 · 박기정 · 강빈구 · 김영민 *
Address 연세대학교 이과대학 생물학과
Bibliography Korean Journal of Microbiology, 29(6),387-391, 1991
Key Words Methylobacterium extorquens AM1, Methanol, Polyamine, Methylotroph
Abstract Methylobacterium extorquens AM1 growing on methanol as a sole source of carbon and energy was found to grow most rapidly (t_d=6h) at 30.deg.C in a mineral medium (pH 7.0) containing 0.5% (v/v) methanol which was agitated at 200 rpm (optimal cultivation condition). Cells grown under the optimal cultivation condition contained more spermidine, but less putrescine, than the cells grown on 2.5%(v/v) (t_d=8h) or at 20℃ (t_d=8h). Cells cultivated under the optimal condition was found to contain more spermidine than the cells grown at pH 6.0 (t_d=7h) and pH 8.0 (t_d=7.3h). The cells growing at the stationary phase under the optimal condition accumulated more spermine or putrescine than the cells growing at the same phase on 2.5%(v/v) methanol or at pH 6.0 and pH 8.0, respectively. M. extorquens AM1 grown in a medium agitated at 100 rpm (t_d=8.8h) contained less spermidine and spermine than the cells grown under the optimal cultivation condition.
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