| Title | Multicopy streptomyces 플라스미드 pJY711의 재조합 유도체의 특성 | ||
| Author | 염도영 · 공인수 · 유주현 | ||
| Address | 연세대학교 공과대학 식품공학과 | ||
| Bibliography | Korean Journal of Microbiology, 28(1),35-40, 1990 | ||
| DOI | |||
| Key Words | Streptomyces, plasmid vector, broad host range | ||
| Abstract | The restriction clevage map of multi-copy recombinant plasmid, pJY712(8.1kb), carrying the thiostrepton resistance gene(tsr) was determined. pJY712 had a broad host range in Streptomyces and contained single BglII site for cloning purpose. The plasmid showed the phenomenon of lethal zygosis (Ltz^+). Transformation frequency of pJY712 was 5.0×10^4 transformants per ug plasmid DNA (TFU) in S. lividans. Plasmid pJY713 was constructed by inserting the tyrosinase gene(mel) into the BclI site of pJY712. Recombinant plasmid pJY714 carrying the mel gene was constructed by in vitro deletion of a segment (1.9kb BglII-BclI fragment) from pJY713. | ||
| Download PDF | Kor_280106_35-40p.pdf | ||