| Title | Brevibacterium lactofermentum의 원형질체 융합에 의한 유전자 재조합 | ||
| Author | 이혜경 · 최순영 · 윤윤경 · 이영하 · 민경희 | ||
| Address | 숙명여자대학교 이과대학 생물학과; 충남대학교 미생물학과 | ||
| Bibliography | Korean Journal of Microbiology, 28(2),98-103, 1990 | ||
| DOI | |||
| Key Words | Protoplast fusion, genetic analysis of recombinats, Brevibacterium lactofermentum | ||
| Abstract | Brevibacterium lactofermentum SWA (arg trp) and B. lactofermentum SWB (met ser) were obtained from UV and NTG treatment. The rates of protoplast formation by B. lactofermentum SWA and SWB were 99.93% and 99.98%, respectively when each strain was treated with penicillin G in mid exponential growth phase, followed by incubation with 400 ug/ml of lysozyme in lysis fluid supplemented with 0.4M sucrose. Frequencies of protoplast regeneration in B. lactofermentum SWA and B. lactofermentum SWB were 9.27% and 10.32% respectively, on regeneration medium containing 0.5M sodium succinate, 50 mM Mg^2+, and 3% PVP. In intraspecific protoplast fusion between B. lactofermentum SWA and B. lactofermentum SWB, fusion frequency of 2.30×10^-5 was observed by using the 100mM CaCl_2 and 30% PEG 6,000 in fusion fluid. Relative recombinant frequencies in each marker by means of selective media could be used for genetic analysis. | ||
| Download PDF | Kor_280202_98-103p.pdf | ||