| Title | Agrobacterium tumefaciens의 염색체 DNA내에 존재하는 종양 유발 지역의 클로닝 | ||
| Author | 심웅섭1 · G.A. Cangelosi2 · E.W. Nester2 | ||
| Address | 1고려대학교 생물학과; 2워싱톤대학교 미생물학과 | ||
| Bibliography | Korean Journal of Microbiology, 28(2),104-108, 1990 | ||
| DOI | |||
| Key Words | A. tumefaciens, virulence region, Cloning | ||
| Abstract | The chromosomal DNA of Agrobacterium tumefaciens contains the genes required for bacterial attachment to plant cell which is an essential stage in crown gall tumorigenesis by Ti-plasmid. In order to clone the genes, Agrobacterium tumefaciens strain A5512 was mutagenized by transposon Tn5 and two Agrobacterium tumefaciens mutants which are attachment-defective and nontumorigenic were isolated. From one of the two mutants, a chromosomal virulence region which was required for attachment to the plant cells was cloned. | ||
| Download PDF | Kor_280203_104-108p.pdf | ||