| Title | Saccharomyces cerevisiae에서 효모 superkiller 유전자(SK13)의 발현 | ||
| Author | 이상기 · Reed B. Wickner * | ||
| Address | 한국과학기술연구원 유전공학센터; * 미국 NIH | ||
| Bibliography | Korean Journal of Microbiology, 28(2),114-119, 1990 | ||
| DOI | |||
| Key Words | SKI3, S. cerevisiae, lacZ, gene fusion | ||
| Abstract | A yeast chromosomal superkiller gene (SK13) was cloned and expressed in ski^- Saccharomyces cerevisiae strains. The gene was fused to the structural region of E. coli lacZ gene at its C-terminus in a yeast-E. coli shuttle vector, pSR605. The fused gene complemented ski3^- strains with SK13 activity and the quantitative level of expression was measured as determined by assaying ?galactosidase activity. The SDS-polyacrylamide gel electrophoresis and the Western blot analysis of this fused protein showed the immuno-reacted bands with a protein of the estimated molecular size (ca.250Kd). | ||
| Download PDF | Kor_280205_114-119p0.pdf | ||