| Title | 원형질체 융합 방법을 이용한 전분 발효성 효모의 개발 | ||
| Author | 전순배 · 배석 · 이기영 * · 김수현 ** · 이진종 ** · 김상문 *8 | ||
| Address | 전남대학교 미생물학과; * 공업화학과; ** 생물학과 | ||
| Bibliography | Korean Journal of Microbiology, 28(2),120-127, 1990 | ||
| DOI | |||
| Key Words | Filobasidium capsuligenum, Saccharomyces cerevisiae, protoplast fusion | ||
| Abstract | Intraspecific fusion frequency between Filobasidium capsuligenum CBS6122-2 ade and met trp or met strains was 2.9×10^-3 and 8.5×10^-3, respectively. And intergeneric fusion frequency between Folobadidium capsuligenum CBS 4318 (cys his) and Saccharomyces cerevisiae 262-12-1 (hom3 thr1) or X2180-1A (ade thr) was 8.8×10^-6 and 9.5×10^-4, respectively. Nuclear fusion appears to occur in fusants between intraspecies and intergenera as strongly suggested by DNA content, nuclear staining, comparison of survival rate to UV light and mitotic segregation analysis. It was also found that α-amylase and glucoamylase activity from intraspecific hybrids was 1.6-2.1 fold increased when compared with that from their parents. | ||
| Download PDF | Kor_280206_120-127p.pdf | ||