| Title | Klebsiella aerogenes ATCC 10031의 카드뮴 해독기작 | ||
| Author | 이기성 · 유순애 · 곽인영1 · 박영식2 · 최영길2 | ||
| Address | 배재대학교 생물학과; 1유전공학과; 2한양대학교 자연과학대학 생물학과 | ||
| Bibliography | Korean Journal of Microbiology, 28(2),134-144, 1990 | ||
| DOI | |||
| Key Words | cadmium, detoxification, Cd:S complex, polymeric Cd:Pi complex in vivo ^31P NMR | ||
| Abstract | In order to examine that what kind of system correlated with cadmium detoxification mechanism in Klebsiella aerogenes ATCC 10031, we tried to investigate the effect of phosphate upon the detoxification and also elucidate whether the cadmium phosphate and/or polymeric Cd-Pi complex is formed actually in cell or not. As the results, it was shown that growing pattern had long lag adaptive phase of 12 hr to 24 hr, at the concentrations of 0.02 mM and 0.08 mM cadmium, respectively. Cadmium was accumulated more highly in the fraction of cell wall and membrane than in those of cytoplasm. In case of phosphate starving cells added cadmium, inorganic polyphosphate system was primarily correlated with Cd-detoxification during the lag phase for the accommodation to cadmium, on the other hand, Cd:Sulfide complex system secondarily correlated it during the stationary phase. These results implied that polyphosphate system and Cd:sulfide complex system, these two systems were operated compensatively each other. Considering the results obsdrved with EM and examined tha changes of sulfide and polyphosphate amount, it was reflected that Cd:S complex was located at the cell surface. In the results of in-vivo ^31PNMR spectra in the cells with cadmium pressure, several phosphate signals arose newly from the polyphosphate region with moving chemical shift of it. This phinomenon strongly implied the actual existence of Dd:Pi comples and /or Cd:poly-P complex in the cell and also the cellular compartmentalization of cadmium detoxifying mechanism. | ||
| Download PDF | Kor_280208_134-144p.pdf | ||