| Title | L-페닐알라닌 대량생산을 위한 재조합 플라스미드 구성 | ||
| Author | 이새배 · 박청 · 원찬희 · 최덕호 · 임번삼 | ||
| Address | (주)미원 중앙연구소 | ||
| Bibliography | Korean Journal of Microbiology, 28(2),169-173, 1990 | ||
| DOI | |||
| Key Words | L-phenylalanine, recombinant plasmid, enzyme avtivity | ||
| Abstract | For the overproduction of L-phenylalanine using Escherichia coli, the authors constructed various recombinant plasmids including pMW 10, pMW 11 and pMW 12. The aroF^FR and pheA^FR genes for the production of L-phenylalanine were isolated from Escherichia coli MWEC 101-5 strains. The productivity and atability of Escherichia coli regulatory mutants containing recombinant plasmids were investigated to evaluate the efficiency of the aroF^FR and pheA^FR genes. The MWEC 101-5/pMW 11 strain produced 24.3g/l of L-phenylalanine while its stability was 73.8 percent. The specific activity of prephenate dehydratase in the MWEC 101-5/pMW 11 strain increased by 26-fold compared with that of Escherichia coli K-12. | ||
| Download PDF | Kor_280213_169-173p.pdf | ||