| Title | Escherichia coli K-12 대사조절 변이주에 의한 L-페닐알라닌 생산 | ||
| Author | 이새배 · 박청 · 원찬희 · 최덕호 · 임번삼 | ||
| Address | (주)미원 중앙연구소 | ||
| Bibliography | Korean Journal of Microbiology, 28(2),174-179, 1990 | ||
| DOI | |||
| Key Words | L-phenylalanine, regulatory mutant, enzyme activity | ||
| Abstract | In order to overproduce L-phenylalanine, various kind of regulatory mutants were isolated from parental Escherichia coli K-12. MWEC 83 Producing 7.4g/l of L-phenylalanine has been derived as a tyrosine and tryptophan double auxotrophic mutant. To produce L-phenylalanine without adding L-tyrosine and L-tryptophan, revertant strain MWEC 101 was isolated from MWEC 83. Further various analogues and valine resistant mutants were isolated from MWEC 101. MWEC 101-5 was the most excellent strain that produced 17.9g/l of L-phenylalanine after having been cultivated for 54 hours in 15% glucose medium. It was disclosed that activities of rate-limiting enzymes including chorismate mutase and prephenate dehydratase in MWEC 101-5 were desensitized to 2mM L-phenylalanine in the enzyme reaction mixture and that activities level of 3-deoxy-D-arabino-heptulosonic acid-7-phosphate synthase and prephenate dehydratase were increased more than 20 times over those of the parental strain. | ||
| Download PDF | Kor_280214_174-179p.pdf | ||