| Title | Glucoamylase 유전자 STA를 포함한 재조합 플라스미드들의 Saccharomyces cerevisiae에서의 발현 | ||
| Author | 박장서 · 박용준 · 이영호 · 강현삼 * · 백운화 | ||
| Address | 동양맥주 두산연구소 · * 서울대학교 미생물학과 | ||
| Bibliography | Korean Journal of Microbiology, 28(3),181-187, 1990 | ||
| DOI | |||
| Key Words | STA gene, S. cerevisiae, industrial yeast strain | ||
| Abstract | STA gene coding glucoamylase was introduced into haploid Saccharomyces cerevisiae SHY3 and polyploid Saccharomyces cerevisiae 54. We constructed the recombinant plasmid by substituting the promoter region of alcohol dehydrogenase isoenzyme I gene for that of STA gene to increase the expression of STA gene and found that the activity of glucoamylase was increased in transformants. The plasmid stability was improved remarkably when we got the STA gene into the plasmid which had centromere. The activity of glucoamylase and transformation frequency of it, however, was decreased because of low copy number. Industrial polyploid strain was transformed with the recombinant plasmid having the 2u origin of replication and STA gene. It produced more alcohol than host when fermented in liquefied starch media. The industrial strain, however, was not transformed with the autonomously replicating plasmid containing centromere. | ||
| Download PDF | Kor_280301_181-187p.pdf | ||