| Title | 돌연변이와 DNA 손상회복에 미치는 muc 유전자의 기능 | ||
| Author | 전홍기 · 이상률 · 백형석 | ||
| Address | 부산대학교 자연과학대학 미생물학과 | ||
| Bibliography | Korean Journal of Microbiology, 28(3),192-198, 1990 | ||
| DOI | |||
| Key Words | pMK101, pSL4, muc gene, umu gene, DNA repair | ||
| Abstract | To determine whether the muc region of pKM101 and its mutant pSL4 is sufficient for the expression of these phenotypes, muc regions of pKM101 and pSL4 were subcloned onto the highcopy number vector pKB354 and were selected the cloned pJB200 and pJB210. The recombinant plasmids pJB200 and pJB210 were introduced into umu C36^- uvr A6^-(TK610) strain and determined the protection effect and mutagenecity for UV and MMS. The protection effect and mutagenecity of umu C36^-uvr A6^-(TK610) were supressed by muc gene of the recombinant plasmids. The muc gene of pSL4 has higher effect than that of pKM101. The recombiant plasmid pJB210(inclued muc gene of pKM101) did not affect uv-mutagenesis in the recA^-(JC2926) mutant. | ||
| Download PDF | Kor_280303_192-198p.pdf | ||