| Title | Methylumbelliferyl 형광기질을 이용한 평판배지상의 미생물 체외 세포효소측정방법 | ||
| Author | 김상진 · H.-G, Hoppe * | ||
| Address | 한국과학기술연구원 유전공학센터 | ||
| Bibliography | Korean Journal of Microbiology, 28(3),229-235, 1990 | ||
| DOI | |||
| Key Words | Microbial extracellular enzyme detection, Methylumbelliferyl-substrates, Agar plate | ||
| Abstract | A rapid and sensitive method to detect the extracellular enzymatic activity of bacteria colonies grown on agar plates is described. Selective agar media supplemented with protein, starch, chitin, Tween-80, etc. are conventionally used to detect biochemical properties of bacteria. It has been experimentally demonstrated with bacteria pure cultures that fluorogenic Methylumbelliferyl (MUF)-substrates are excellent substrate analogues for normally occurring polymers. Based on MUF-substrate hydrolysis the new method provides reliable qualitative estimates of extracellular enzymatic properties of bacteria within minutes using pure cultures as well as agar plates prepared for colony counts. Extracellular enzyme activities of heterotrophic bacteria from freshwater ecosystems and marine sediment using this method are discussed. | ||
| Download PDF | Kor_280308_229-235p.pdf | ||