Title Streptomyces viridochromogenes의 ascorbic acid 산화 활성
Author 김성욱 · 노재영 · 김재헌
Address 단국대학교 자연과학대학 미생물학과
Bibliography Korean Journal of Microbiology, 28(4),283-289, 1990
DOI
Key Words Streptomyces viridochromogenes, aerial mycelium formation, ascorbate oxidation
Abstract The competence time of Streptomyces viridochromogenes for aerial mycelium formation was determined. Within 10 hrs after spore inoculation the submerged mycelium was programed to form aerial mycelium, when the former was laid on agar plate. The white aerial mycelium was formed 17-22 hrs after the transfer. Ascorbic acid oxidizing enzyme band on native gel showed chracteristic mobility change during aerial mycelium formation. Total activity of this enzyme did not show any correlation with the differentiation. The asay condition for the crude enzyme was determined. EDTA and FeCl_2 showed stimulatory effect. Approximate ratio of oxygen consumed to ascorbic acid oxidized was 1:1.
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