Title 호수 생태계에서 세균 생산량 측정 방법의 비교
Author 김명운 · 강찬수 · 김상종
Address 서울대학교 자연과학대학 미생물학과
Bibliography Korean Journal of Microbiology, 28(4),318-323, 1990
DOI
Key Words Bacterial production, ^3H-thymidine incorporation rate, ^3H-leucine, Lake Soyang
Abstract The bacterial secondary production was measured at 6 sites of Lake Soyang in October, 1989 by ^3H-thymidine incorporation rate. Verification for the method of bacterial secondary production measurement showed that ^3H-thymidine incorporated into DNA, RNA and protein by average percentage of 38.45, 42.27 and 20.07%, respectively. THe more increased incoporated ^3H-thymidine, the more increasde DNA fraction, but protein fraction was generally low. Incorporation of rate of ^3H-thymidine. ^3H-leucine into protein correlated with protein fraction of incorporated ^3H-thymidine. Conversion factors were calculated as follows; 1.83×10^20 cells/moles of thymidine incorporated/hr and 1.69×10^22 cells/moles of leucine incorporated/hr.
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