| Title | SV 40 promoter를 갖는 plasmid에 의한 NIH3T3 섬유아세포의 형질전환 | ||
| Author | 이영환 · 김광식 · 서용택 · 김용웅 · 박남용 * · 황태주 ** | ||
| Address | 전남대학교 농과대학 농화학과; * 수의대학 수의학과; ** 의과대학 소아과 | ||
| Bibliography | Korean Journal of Microbiology, 27(1),10-15, 1989 | ||
| DOI | |||
| Key Words | Oncogene, tumorigenicity, SV40 Promoter, Cell Transformation | ||
| Abstract | The plasmid pKOneo, containing SV40 transcriptional promoter, has been used in the mouse tumorigenicity assay for oncogene studies. This assay employs a cotransfection of NIG3T3 fibroblast cells with the desired DNA and the plasmid pKOneo. This oncogene assay, however, has been speculated due to the SV40 transcriptional promoter in the plasmid pKOneo. This research was designed to investigate if the plasmid pKOneo alone is capable of transforming NiH3T3 fibroblast cells. The NIH3T3 subclones were established after the NIH3T3 cells were transfected with the plasmid pKOneo alone. The estabilished NIH3T3 subclones, containing the exogeneous plasmid pKOneo in their chromosomes, were examined for their expression of transformation-associated parameters. The results indicate that this plasmid pKOneo alone has positive effects on transformation of NIH3T3 cells after integration into cellular chromosomes. | ||
| Download PDF | Kor_270102_10-15p.pdf | ||