| Title | 효모에서 plasmid의 복제에 대장균 plasmid DNA가 미치는 영향에 관한 연구 | ||
| Author | 김태국 · 최철용 · 노현모 | ||
| Address | 서울대학교 동물학과 분자유전학 연구실 | ||
| Bibliography | Korean Journal of Microbiology, 27(1),16-20, 1989 | ||
| DOI | |||
| Key Words | E. coli plasmid DNA sequences, yeast plasmid, replication | ||
| Abstract | The effect of E. coli plasmid DNA sequences contained by chimeric vectors on plasmid replication was investigated. We constructed YRp7- or 2㎛ circle-based plasmids containing E. coli plasmid DNA sequences and those not containing it. By examining their maintenance in yeast, we showed that plasmid without E. coli plasmid DNA sdquences was nore stable and presented higher copy number, and espressed higher level of hepatitis B viral surface antigen as a foreign gene. This result suggested that E. coli plasmid DNA sequences within chimeric plasmid somehow inhibited plasmid replication in yeast. | ||
| Download PDF | Kor_270103_16-20p.pdf | ||