| Title | Klebsiella pneumoniae의 nif Gene Cluster의 Cloning 및 Escherichia coli 에서의 발현 | ||
| Author | 이재선 · 이성희 · 심웅섭 | ||
| Address | 고려대학교 이과대학 생물학과 | ||
| Bibliography | Korean Journal of Microbiology, 27(1),21-26, 1989 | ||
| DOI | |||
| Key Words | nif genes, cloning, transformation, expression | ||
| Abstract | The chromosomal DNA of Klebsiella penumoniae KNF1 was partially digested with HindIII. pBR322 was completely digested with same enzyme with additional alkaline phosphatase treatment. Both DNA samples were ligated and transformed into E. coli KO60. Single coloneis of the transformed cells were isolated on N0free agar media. These colonies were ampicillin-resistant and tetracycline-sensitive. When the plasmids in transformants were cured, nitrogen-fixing activities were lost. Therefore, these transformants harbored recombinant plasmid including nif genes of K. pneumoniae. Nitrogenase activity of transformant was higher than or the same as that of K. pneumoniae KNF1. | ||
| Download PDF | Kor_270104_21-26p.pdf | ||