| Title | 효소의 전기영동에 의한 Trichoderma속 균의 종내, 종간 잡종의 동정 | ||
| Author | 민경렴1 · 박희문2 · 하영칠 | ||
| Address | 서울대학교 자연과학대학 미생물학과; 1태평양화학기술연구소 생물공학실; 2Lab. of Biochemical & Metabolism, NIDDK., NIH., Methesda, MD., 20852, USA | ||
| Bibliography | Korean Journal of Microbiology, 27(1),27-34, 1989 | ||
| DOI | |||
| Key Words | electrophoresis, isozyme, intra/interspecific hybrids, activity stain | ||
| Abstract | In order to evaluate the applicability of enzyme electrophoresis for the identification of intra/interspecific hybride obtained by the protoplast fusion in Trichoderma, soluble proteins, intracellular soluble enzymes and extracellular β-glucosidase were analyzed by polyacrylamide gel electrophorsis. As the results, patterns of soluble protein, and isozyme patterns of peroxidase, malate dehydrogenase, and β-glucosidase in hydrids were defferent from those in parental and wild type strains. Therefore, it was established that the analysis of protein pattern by electrophoresis could be applied to isolate and identify the hybrids from the protoplast fusion. | ||
| Download PDF | Kor_270105_27-34p.pdf | ||