| Title | Streptococcus sanguis의 구형 hydroxyapatite 비드에의 부착 assay 방법의 개량 | ||
| Author | 최선진 · 이시영 · 송요한 | ||
| Address | 서울대학교 치과대학 미생물학교실 | ||
| Bibliography | Korean Journal of Microbiology, 27(1),48-55, 1989 | ||
| DOI | |||
| Key Words | Streptococcus sanguis, adherence, hydroxyapatite bead, sample self-absorption | ||
| Abstract | The purpose of the present investigation was to improve several procedures being used in the adherence assay of Streptococcus sanguis cells to hydroxyapatite (HA) beads and to study the effect of the beads on the counting of radioactivity. The standard adhere assay involved the adherence of radiolabeled bacteria to 40mg of HA beads. The beads were mixed with ['H]thymidine-labeled bacterial cells and incubated for 60 minutes at room temperature. Unadsorbed cells were removed, the beads with adsorbed cells were dried, and the radioactivity was monitered in a scintillation spectrometer. The 30 seconds sonication of cells in a form of long chains appeared to be adequate for obtaining mostly singlet or doublet cells. Unlike the counting of S. sanguis cell suspension, bacterial cells adhered to HA or saliva-coated HA(SHA) required smaller volume (2.5ml) of scintillaton fluid for better counting. Eighteen percent quenching of counts could be attributed to the beads. Among 3 procedures commonly used to equilibrate the beads for adherence assay, no differences were found in their effectiveness. The HA beads on which the bacteria remained attached in scintillant during the counting were found to be the source of sample self-absorption representing 34.5% of the total radioactivity counts resulting from the beads dissolved in HCl solution. | ||
| Download PDF | Kor_270108_48-55p.pdf | ||