| Title | 인삼뿌리 썩음 병균 fusarium solane에 대한 streptomyces species의 길항작용 | ||
| Author | 정영륜1 · 오승환2 · 정후섭3 | ||
| Address | 1한국화학연구소 농약활성연구실; 2한국인삼연초연구소 경작시험장; 3서울대학교 농과대학 농생물학과 | ||
| Bibliography | Korean Journal of Microbiology, 27(1),56-62, 1989 | ||
| DOI | |||
| Key Words | Chitinase, Streptomycetes, Fusarium solani | ||
| Abstract | Antagonistic effects of Streptomyces species aganinst Fusarium solani causing ginseng root rot were investigated in terms of chitinase activity and growth inhibition in vitro. Among 131 isolates of streptomycetes obtained from ginseng cultivating soil, 9 isolates producing large clear zone around the colony on a chitin agar medium were selected for further study. All 9 isolates produced chitinase in a range from 0.10 to 0.38 U lysing cells of F. solani and inhibited germination of the conidia. In the ten-fold condentrated culture filtrate of S. alboniger ST59 and S. roseolilacinus ST129, the number of conidia of F. solane was reduced to about 20% of original count within 14 days. When S. alboniger ST59 and F. solani were grown simultaneously in the mineral saly medium, chitinase activity increased with incubation period, whereas mycelial volume of F. solani decreased. In a chitin added mineral salt medium, chitinase activity increased during the first four days and maintained steady level until the 8th day, and increased thereafter. S. alboniger ST59 lysed mycelia, conidia and even chlamydospores of F. solani. It is probable that the antagonistic activity of this streptomycete against F. solani is the lysis of fungal cell wall by streptomycete producing chitinase affected by antifungal substances. | ||
| Download PDF | Kor_270109_56-62p.pdf | ||