| Title | 고정화된 aspergillus phoenicis를 이용한 progesterone 전환 | ||
| Author | 박희은 · 김말남 | ||
| Address | 상명여자대학교 생물학과 | ||
| Bibliography | Korean Journal of Microbiology, 27(1),70-76, 1989 | ||
| DOI | |||
| Key Words | Aspergillus phoenicis, progesterone bioconversion, immobilization | ||
| Abstract | Progestrone bioconversion by immobilized Aspergillus phoenicis was studied. Progesterone was converted into 11α-hydroxyprogesterone and 3-minor byproducts. Whole cells of A. phoenicis were immobillized by enreappment with calcium-alginate, K-carrageenan, or polyacrylamide. Of these materials tested, cell immobilized in Ca^2+ - alginate gels showed the highest activity for 11α-hydroxylation of progesterone. In the case of mycelia immobilized in Ca^2+ - alginate, futher progressing hydroxylation of 11α - hydroxyprogesterone was greatly reduced. Spores of A. phoenicis which were immobillized with Ca^2+ - alginate and germinatedin situ for 25 hours showed higher 11α - hydroxylase activity than those of entrapped whole mycelia and maintained initial enzyme activity for all 8 times of repeated use. After 16 times of reuse, the activity was declined 30% or more. When culture media and Zn^2+ were introduced into the reaction media, the activity of the immobilized mycelia which had been lowered due to many times of reuse was effectively reactivated. | ||
| Download PDF | Kor_270111_70-76p.pdf | ||