| Title | Saccharomyces cerevisiae에서 RNA1 유전자의 클로닝 | ||
| Author | 송영환 * · 고상석 · 이영석 · 강현삼 | ||
| Address | 서울대학교 미생물학과; * 부산수산대학교 미생물학과 | ||
| Bibliography | Korean Journal of Microbiology, 27(2),77-84, 1989 | ||
| DOI | |||
| Key Words | Saccharomyces cerevisiae, splicing, RNA1, cloning | ||
| Abstract | The temperature sensitive (ts) mutation on RNA1 gene of Saccharomyces cerevisiae prevents growth at restrictive temperature (36℃) by accumulation of precursor tRNA, rRNA and mRNA (Hutchison et al., 1969; Shiokawa and Pogo, 1974; Hopper et al., 1978). RNA1 gene was cloned by complementation of the temperature sensitive growth defect of an rna1-1 mutant strain and identified by retransformation and concomitant loss of recombinant plasmid on non-selective condition. By deletion mapping, it was found that RNA1 gene resides within 3.5kb of BglII fragment. | ||
| Download PDF | Kor_2702001_77-84p.pdf | ||