| Title | 소성장호르몬 유전자의 조직 특이성 발현에 미치는 바이러스 enhancer의 영향 | ||
| Author | 박계윤 · 김수미 · 노정혜 | ||
| Address | 서울대학교 자연과학대학 미생물학과 | ||
| Bibliography | Korean Journal of Microbiology, 27(2),85-91, 1989 | ||
| DOI | |||
| Key Words | Enhancers, SV40, MCMV, bovine growth hormone, tissue-specific expression, CAT assay | ||
| Abstract | The effect of SV40 and murine cytomegalovirus (MCMV) enhancers on the general and tissue-specific gene expression was investigated. Recombinant plasmids containing these transcriptional engancers downstream of a structural gene for chloramphenicol acetyl transferase (CAT) were constructed. The transient expression of CAT gene from these plasmids was measured in monkey (CV1PD) and HeLa cells. Both SV40 and MCMV engancers activated the expression of CAT gene by more than 20 and 150 folds, respectively, compared with engancerless plasmids. When the SV40 promoter, upstream of CAT gene, was replaced with 2.2 kbp of promoter regulatory region of bovine growth hormone (bGH) gene, there was no expression of CAT even in the presence of enhancers, reflecting the tissue-specific expression of bGH genes. However, when the bGH regulatory region was shortened to 230 bp, the expression level increased dramatically in the presence of SV40 enhancers. In contrast, the expression from the shortened promoter was only marginally activated by the stronger MCMV enhancer. | ||
| Download PDF | Kor_2702002_85-91p.pdf | ||