| Title | Neurospora crassa로부터 arginine transporter의 순수분리 | ||
| Author | 이연희 · R. L. Weiss | ||
| Address | 캘리포니아 주립대학 화학 및 생화학과 | ||
| Bibliography | Korean Journal of Microbiology, 27(2),117-123, 1989 | ||
| DOI | |||
| Key Words | Vaculoe, arginine transporter, NBZ arginyl diazomethane | ||
| Abstract | Radioactive N-α-p-nitrobenzoxycarbonyl (NBZ)-L-[2,3-^3H] arginyl diazomethane was used as an affinity label for the vacuolar arginine transporter in Neurospora crassa. Vacuolar matrix proteins were removed by fracturing the membranes with freeze-thaw method in dry ice/ethanol bath. Vacuolar membrane proteins were then wasged with 500mM NaCl to remove ionically bound derivatives and peripheral membrane proteins from vacuolar membranes. After dissolved in 1% Titon X-100, dissolved vacuolar memvrane proteins were separated with molecular sieve column chromatography, anion and cation exchange chromatographies. The arginine transporter was purified giving the purification factor of 1136. | ||
| Download PDF | Kor_2702006_117-123p.pdf | ||