| Title | 플라스미드에 존재하는 lactobacillus casei의 phospho-β-galactosidases 유전자 | ||
| Author | 문경희 · 박정희 · 최순영 · 이유미 · 김태한 * · 하영칠 ** · 민경희 | ||
| Address | 숙명여자대학교 생물학과; 일동제약(주) 중앙연구실 * ; 서울대학교 자연과학대학 미생물학과 ** | ||
| Bibliography | Korean Journal of Microbiology, 27(3),181-187, 1989 | ||
| DOI | |||
| Key Words | Phospho-β-galactosidases gene, plasmid, Lactobacillus casei | ||
| Abstract | Plasmid DNA was isolated from Lactobacillus casei SW-M1(Lac^+ strain). The curing frequencies of pPLac plasmid from L. casei SW-M1 showed 43% for acriflavin treatment and 53% for ethidium bromide treatment after 3 times transfer. On the charaterization of pPLac plasmid, it was found that the plasmid contained gene encoding phospho-β-galactosidase for lactose utilization. Lactose-PTS(phosphotransferase system)was involved in membrane transport system in Lac^+ strain. Induction of phospho-β-galactosidase was specially effective by galactose, lower effect with lactose and glucose but not by IPTG(isopropyl-β-D-thiogalactoside). This result showed that induction of phospho-β-galactosidase by IPTG did not appeared. The catabolite repression of phospho-β-galactosidase synthesis by glucose was not found in L. casei. | ||
| Download PDF | Kor_270303_181-187p.pdf | ||