| Title | Lactobacillus casei의 phospho-β-galactosidase 유전자의 대장균내 분자클로닝 | ||
| Author | 문경희 · 박정희 · 최순영 · 이유미 · 김태한 * · 김연수 ** · 민경희 | ||
| Address | 숙명여자대학교 생물학과; 일동제약(주) 중앙연구실 * ; 서울대학교 자연과학대학 미생물학과 ** | ||
| Bibliography | Korean Journal of Microbiology, 27(3),188-193, 1989 | ||
| DOI | |||
| Key Words | Phospho-β-galactosidase gene, molecular cloning, Lactobacillus casei | ||
| Abstract | Gene for lactose catabolism in Lactobacillus casei SW-M1 was encoded by a 60Kb metabolic plasmid. A derivative of only 10kb, pPlac 15 of recombinant plasmid, was constructed by introducing into pBR322 and was cloned into E. coli using restriction endonuclease Pst I. A 10kb insery DNA in plasmid pBR322 was identified as a gene encoded phospho-β-galactosidase by the determination of enzyme activity. Phospho-β-galactosidase was apparently expressed in E. coli. The enzyme activities of cell-free extract from transformant E. coli HB101 carrying pPLac 15 DNA were not different from that of L. casei as a donor strain on the basis of enzyme properites. However, specific activity of phospho-β-galactosidase in the cloned strain with Lac Y^- phenotype of E. coli HB101 was lower than that in L. casei strain. | ||
| Download PDF | Kor_270304_188-193p.pdf | ||