| Title | Staphylococcus aureus에서 분리된 R-plasmid pSBK203상의 Chloramphenicol acetyltransferase 인자의 염기서열 및 유발성 분석 | ||
| Author | 권동현 · 변우현 | ||
| Address | 강원대학교 자연과학대학 미생물학과 | ||
| Bibliography | Korean Journal of Microbiology, 27(3),194-200, 1989 | ||
| DOI | |||
| Key Words | nucleotide sequences, CAT-gene, Staphylococcus aureus plasmid, minicell | ||
| Abstract | The nucleotide sequence of inducible chloramphenicol acetyl-transferase(CAT) gene isolated from a small plasmid pSBK203 of Staphylococcus aureus was determined. The base sequence shows that structural gene of pSBK203-CAT encodes a protein of 213 amino acids and has a leader region which encodes a short polypeptide of 9 amino-acids in its upstream. │^35S│-Methionine labelled CAT gene product in minicell showed almost same mobility with pC194-CAT of which molecular weight is 24Kdal on polyacrylamide gel electrophoresis. Predicted amino acid sequence of pSBK203-CAT has revealed a high degree of homology with the CATs of pC194 and pC221 than those of cat-86, Tn9 and proteus mirabilis PM13. | ||
| Download PDF | Kor_270305_194-200p.pdf | ||