| Title | Salmonella typhimurium에서 Mu dJ(Km lac) operon fusion을 이용한 산소, 무산소 유도 유전자에 관한 연구 | ||
| Author | 김종선 * · 우영대 * · 박종희 * · 김영권 * · 이인수 * · 박용근 ** | ||
| Address | * 한남대학교 이공대학 생물학과; ** 고려대학교 이과대학 생물학과 | ||
| Bibliography | Korean Journal of Microbiology, 27(3),201-209, 1989 | ||
| DOI | |||
| Key Words | Operonfusion, oxygen-regulated genetic loci, map unit. Salmonella typhimurium | ||
| Abstract | Using Mu dJ(Km lac) operon fusion, several oxygen-regulated genetic loci were identified in Salmonella typhimurium. Nine aerobically inducible(oxi) and thirteen anaerobically inducible(ani) operon fusions were newly identified. Based on the control by oxrA regulatory locus, the ani-lacZ fusions were grouped into two classes: class I loci were regulated by oxrA regulatory locus; class II genes were not affected by this locus. Some of the ani-lacZ fusions had required growth in CAA and LB before they exhibited the inducible phinotype. Most of all ani-lacZ fusions were repressed by nitrate and fumarate. Three of the ani loci were mapped into 59±0.14 map unit (YK114), 64 ± 0.2 map unit(YK120), and 93±0.29 map unit(YK112) by testing the cotransduction frequency, respectively. | ||
| Download PDF | Kor_270306_201-209p.pdf | ||