| Title | Aspergilluis oryzae의 원형질체 융합 | ||
| Author | 이수현 · 이주실 · 이영록 | ||
| Address | 고려대학교 이과대학 생물학과 | ||
| Bibliography | Korean Journal of Microbiology, 27(3),216-220, 1989 | ||
| DOI | |||
| Key Words | Aspergilluis oryzae, fusion | ||
| Abstract | As the bsic study about protoplast fusion of amylolytic fungus Aspergillus oryze and nonamyloytic sugar fermenter, Saccaromyces cerevsisae, the intraspecific protoplast fusion of A. oryzae was carried out and the properties of the obtained fusants were investigated. For protoplast fomation from mycellia of auxotrophs, Novozyme 234 as lytic enzyme was the most effective and optimal pH was determined to be pH 5.5-6.0. When the two types of protoplasts were treated with a fusogen including 30% PEG4000, they fused effectively and most of fusants were heterokaryons. Protoplasts aggregated with 30% PEG4000 after fusion treatment were observed by the microscope. Protoplast regeneration frequency was 1.46 to 13.8% and complementation frequency of fusion was 0.12 to 0.16. Fusant strains had a 1.5-fold DNA content compared to that of parent strain. And amylase activity was intermediate between those of parent strains. | ||
| Download PDF | Kor_270308_216-220p.pdf | ||