| Title | 사상균과 효모의 세포융합에 의한 녹말로부터의 에탄올 생산 | ||
| Author | 이주실 · 이수연 · 이영록 | ||
| Address | 고려대학교 이과대학 생물학과 | ||
| Bibliography | Korean Journal of Microbiology, 27(3),221-224, 1989 | ||
| DOI | |||
| Key Words | Aspergillus oryzae, Saccharomyces cerevisiae, fusion, alcohol production | ||
| Abstract | Amylolytic filamentous fungus, Aspergillus oryzae and nonamylolytic sugar fermentable yeast, Saccharomyces cerevisiae were fused by protoplast fusion in order to develope microorganisms having their intergrated function. Aminoacid auxotrophic properties were used as a genetic marker of protoplast fusion, and 35% PEG 4000 was used as a fusogenic agent. Complementation frequengy of fusion was 4.6×10^-6 Obtained fusants showed the morphology of yeast strains, the amylase activity and the ethanol productivity. Among the properties of the fusants, morphology and prototrophic property were sustained stably but their ethanol productivity from starch was reduced. Although fusant strains had 0.5-fold ethanol productivity compared to that of S. cerevisiae in glucose medium, they produced ethanol from strach by direct fermentation. | ||
| Download PDF | Kor_270308_221-224p.pdf | ||