Title 한국에서 분리된 전염성 췌장괴저 바이러스의 새로운 혈청형에 대한 유전자 분석
Author 이정진 · 박정우 · 하영칠 · 정가진
Address 서울대학교 자연과학대학 미생물학과
Bibliography Korean Journal of Microbiology, 27(3),231-236, 1989
DOI
Key Words Serotype, pUC19, cDNA cloning, IPNV, DRT
Abstract The larger segment of double stranded RNA genome from a new serotype of Infectious Pancreatic Necrosis Virus (IPNV), DRT, has been partially cloned at Sma I site in pUC19 and compared with the restriction maps of VR-299 and Sp. Restriction sites found in DRT was distinct and hence a new serotype. The cDNA clones of DRT were about 800, 850, and 1,400 bp long each and do not share any common restriction site. It is not clear yet if there exist any overlapping sequences among them. This partial cloning, however, was sufficient for the comparison of restriction maps with the other serotypes.
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