Title Sodium dodecylbenzenesulfonate(soft type)를 분해하는 미생물의 분리 및 특성
Author 전홍기 · 안영희 · 백형석
Address 부산대학교 자연과학대학 미생물학과
Bibliography Korean Journal of Microbiology, 27(3),279-284, 1989
DOI
Key Words Sodium dodecylbenzenesulfonate(SDBS), Biodegradation, Klebsiella sp., plasmid
Abstract Macroorganisms capable of utilizing sodium dodecylbenzenesulfonate(SDBS, soft type) as a sole carbon source were isolated from nature by using SDBS agar plate technique. Isolated bacteria were examined primarily for biodegradation ability of SDBS, and followed by testing for resistance to several kinds of metal compounds and antibiotics. Among them(152 strains), one strain showed a excellent SDBS-degrading ability with a resistance to amipicillin and rifampicin was selected. This bacterium was identified as Klebsiella sp. and harbored two plasmids of about 4 and 5 kilobases. SDBS-degrading ability was lost when the plasmids were cured by mitomycin C. It was revealed that the degradation of SDBS was controlled by the plasmid DNA encoding genes. The two plasmids were stably maintained in Escherichia coli C600.
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