Title 고온 및 저온처리와 자외선조사에 의한 Campylobacter jejuni의 살균효과
Author 김치경 · 임선희 · 윤만석 · 오학식 · 조민기 *
Address 충북대학교 자연과학대학 미생물학과; * 한림대 의대 미생물학교실
Bibliography Korean Journal of Microbiology, 27(3),291-296, 1989
DOI
Key Words Campylobacter jejuni, disinfection, heat- and cold-treatment, UV-irradiation
Abstract Campylobacter jejuni was studied for its disinfection by heat-and cold-treatment and UV-irradiation. When C. jejuni was treated by heat, no viable cell was found after 10 min treatment at 55℃, whereas small fraction of cell population was survived after 60 min treatment at 45℃ and 50℃. When they were treated by cold temperature for 30 days, no cell was survived at -23℃, but about 4 log of the cells were survived at both temperature of 4℃ and -40℃. When the organisms were UV-irradiated, their survival rates were proportionally varied to the distance of irradiation. The scanning electron microscopic studies of C. jejuni cells treated by the disinfecting agents revealed that shapes of the cells were deformed from spiral rod into spherical form. The heat-treated cells showed rough and damaged surface on the scanning electron micrographs. In the heat-treated cells, some proteins of high molecular weight appeared to become accumulated in the electrophoretic analysis. The DNAs extracted from the cells treated with the physical agents showed some differences in agarose gel electrophoresis, comparing those of normal cells.
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