Title Pseudomonas syringae pv. tabaci가 생산하는 tabtoxin의 미생물학적 검색방법에 관한 연구
Author 백형석 · 구재관 · 전홍기
Address 부산대학교 자연과학대학 미생물학과
Bibliography Korean Journal of Microbiology, 27(3),310-315, 1989
DOI
Key Words Tabtoxin, Pseudomons syringae pv. tabaci, Growth inhibition zone
Abstract Tabtoxin produced in Pseudomonas syringae pv. tabace irreversibly inhibits its known physiological target, glutamine synthetase so that causes wildfire disease on leaves of host plant. In this study, we examined a rapid and sensitive microbiological method for tabtoxin assay in several media. In minimal A agar medium and minimal glucose agar medium, growth inhibition zone of Agrobacterium tumefaciens was larger than that of other indicator strain. However, mostly, growth inhibition zone of indicator strains on the minimal glucose agar medium was smaller than that of on the minimal A agar medium. In complex agar medium, growth inhibition zone was not observed in all the tested indicator strains. Pseudomonas syringae pv. tabaci produced more tabtoxin according to the incubation time. When glutamine was added to the minimal glucose agar medium, growth inhibition zone of Agrobacterium tumefaciens was reduced.
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