| Title | 대장균에서의 Tn5 단백질 증폭생합성 및 안정성 | ||
| Author | 정재성 * · 정재훈 | ||
| Address | 한국과학기술원 과학기술대학 생물학과; * 순천대학 생물학과 | ||
| Bibliography | Korean Journal of Microbiology, 27(4),323-333, 1989 | ||
| DOI | |||
| Key Words | Tn5 polypeptides, IS50, transposase, protein stability, tryptic peptides | ||
| Abstract | Plasmid DNA molecules containing strong promoter upstream from IS50L or IS50R, the two insertion sequences that flank Tn5, were constructed to amplify the synthesis of Tn5-encoded polypeptides. When proteins made by cells that contain these plasmids were analyzed on polyacrylamide gels, enhanced synthesis of IS50R polypeptides could be detected. Synthesis of this polypeptide apparently is initiated within the large open reading frame of this element. In addition, the stability of IS50L-and IS50R-encoded polypeptides was analyzed. It was found that IS50L polypeptides are relatively unstable in vivo. This instability could account for the observed inability of this element to promote transposition. | ||
| Download PDF | Kor_270402_323-333p.pdf | ||