| Title | Pseudomonas cepacia의 전달성 TOL plasmid의 특성과 불화합성 | ||
| Author | 조병남 · 조인선 · 최순영 · 유재근 * · 민경희 | ||
| Address | 숙명여자대학교 이과대학 생물학과; * 국립환경연구원 임호연구소 | ||
| Bibliography | Korean Journal of Microbiology, 27(4),334-341, 1989 | ||
| DOI | |||
| Key Words | Pseudomonas cepacia, Transmissible TOL plasmid | ||
| Abstract | Toluate degradative plasmid from Pseudomonas cepacia SUB37 was determined to be molecular weight as 79.3×10^6(119kb) by agarose gel electrophoresis. It was confirmed that TOL plasmid was transmissible into several strains of Pseudomonas and other genera which were unable to utilize m-toluate. Catechol-2, 3-oxygenase activity which plays an important role in m-toluate biodegradation was determined from crude extracts of P. cepacia SUB37 and transconjugants, suggesting that, as shown for P. putida mt-2, this result provides evidence that the genes coding for the catabolism of m-toluatevia the meta-pathway are also plasmid encoded. A new and independently isolated TOL plasmid from P. cepacia SUB37 belongs to the IncP-4 incompatibility group and could be derivatives of TOL plasmid from P. putida, which is IncP-9 group incompatibility. | ||
| Download PDF | Kor_270403_334-341p.pdf | ||