| Title | Pellicularia fillamentosa로부터 steroid 11β-hydroxylase의 유도 | ||
| Author | 김말남 · 김영숙 | ||
| Address | 상명여자대학교 자연과학대학 생물학과 | ||
| Bibliography | Korean Journal of Microbiology, 27(4),366-372, 1989 | ||
| DOI | |||
| Key Words | Pellicularia fillamentosa, Reichstein's substance S, 11β-hydroxylase, enzyme induction | ||
| Abstract | Twenty-one strains were tested for 11β-hydroxylation of Reichstein's substance S. Four fungi exhibited ability for the reaction, among which Pellicularia fillamentosa showed the highest activity. The 11β-hydroxylase of this fungus was proved to be induced by the substrate, cycloheximide reducing significantly the activity of the enzyme. Range of optimum pH for the 11β-hydroxylation was broad and found to be 2.0-8.0. Test of the enzyme activity at different growing stages, from spore to mycelia, showed that the branching stage of hyphae and the mature mycelial stage were the most effective for the Reichstein's substance S transformation. However, 11β-hydroxylase in the intact spore was turned out to be uninducible with the substrate. | ||
| Download PDF | Kor_270407_366-372p.pdf | ||