| Title | Killer 효모의 원형질체 형성 및 융합조건 | ||
| Author | 정기택 · 방광웅 · 송형익1 · 김재근2 · 정용진 | ||
| Address | 경북대학교 농과대학 식품공학과; 1대구공업전문대학 식품공업과; 2계명실업전문대학 식품영양과 | ||
| Bibliography | Korean Journal of Microbiology, 27(4),422-429, 1989 | ||
| DOI | |||
| Key Words | Killer yeast, Protoplast fusion, Regeneration | ||
| Abstract | Auxotrophic mutant were isolated from wild types by the treatment with NTG as a mutagen, and the conditions of protoplast formation for them were established. The protoplasts of killer yeast Saccharomyces cerevisiae K52 were formed to the level of above 70% when cells grown for 20 hr in PM medium were treated with 200 unit/ml Lyticase 50,000 at 30℃ for 60 min after pretreatment of 50 mM 2-mercaptoethanol in 10mM potassium phosphate buffer (pH 7.5) containing EDTA and 0.6 M sorbitol for 15 min. Also, the protoplast of the recipient S. cerevisiae S 29 were formed to the level of above 85% as it was cultured to the log phase of 24 hr in PM medium under the same conditions. The fusion frequency between the protoplast of killer yeast S. cerevisiae K 52 and the protoplast of recipient S. cerevisiae S 29 was reached to 8.2×10^-6 when the hypertonic regeneration medium embeded with the fused protoplasts after mixing the parental protoplasts to 10^8 cells/ml in SP buffer containing 20 mM CaCl_2 and 30% PEG 6,000 for 15 min at 30℃ were incubated. | ||
| Download PDF | Kor_270415_422-429p.pdf | ||