| Title | Bacillus macerans의 BmaI endonuclease의 특성에 대한 연구 | ||
| Author | 권용태 · 전희숙 · 노현모 | ||
| Address | 서울대학교 자연과학대학 동물학과 분자유전학실 | ||
| Bibliography | Korean Journal of Microbiology, 26(1),1-5, 1988 | ||
| DOI | |||
| Key Words | type II restriction endonuclease, BmaI endonuclease, Bacillus macerans | ||
| Abstract | The isolation and characterization of a new type II restriction endounclease, BamI, from Bacellus macerans ATCC 8244 were described. BmaI endonuclease was partially purified by procedures of ammonium sulfate fractionation, DEAE-cellulose and phosphocellulose chromatographies. This enzume recognized one site on pBR322 DNA, two sites on Bluescribe DNA, three sites on λDNA and no site on SV 40 DNA. The same cleavage patterns for vareius DNAs as PvuI indicated that BamI is an isoschisomer of PvuI whose recognition sequence is 5'-CGATCG-3'. The optimal pH for the BmaI endonuclease activity was about 7.0 and optimal NaCl concentration was about 100mM. Manganese ion could partially replace magnesium as a cofactor, but calcium could not at all. | ||
| Download PDF | Kor_260101_1-5p.pdf | ||