| Title | Cellulomonas biazotea cellobiase gene의 대장균에의 형질발현 | ||
| Author | 박영길 · 연창석 · 최영길 | ||
| Address | 한양대학교 유전공학과 | ||
| Bibliography | Korean Journal of Microbiology, 26(1),6-12, 1988 | ||
| DOI | |||
| Key Words | Cellulomonas biazotea, Cellobiase gene, Gene expression | ||
| Abstract | Cellobiase (β-glucosidase) is an enzyme of the cellulase system in cellulolytic microor-ganisms. The chromosomal DNA fragment which include cellobiase gene of Cellulomonas biazotea was cloned in Eschericia coli via plasmid pBR 322 vector. Restriction enzyme Sal I was used to obtain adequate size of fragments from C. biazotea. chromosomal DNA. The transformant of E. coli HB101 with recombinant plasmid pBG101 showed cellobiase activity, which is not ordinary in E. coli HB101. The enzyme activity of the transformant was as of 20% lower than that of C. biazotea. | ||
| Download PDF | Kor_260102_6-12p.pdf | ||