| Title | Brevibacterium flavum의 glutamate dehydrogenase결핍돌연변이주의 분리 및 특성 | ||
| Author | 최순영 · 성하진 * · 민경희 | ||
| Address | 숙명여자대학교 이과대학 생물학과; * 고려대학교 농과대학 유전공학과 | ||
| Bibliography | Korean Journal of Microbiology, 26(2),93-100, 1988 | ||
| DOI | |||
| Key Words | Characterization of gdh^- mutant, nitrogen assimilation, regulation of GDH, GS and GOGAT synthesis | ||
| Abstract | In order to understand the regulation of glutamate dehydrogenase(GDH) synthesis in Brevibacterium flavum, we have isolated a mutant lacking NADP-linked GDH activity by ethlmethane sulfonate treatment. The gdh^- mutant was grown on the minimal plate with 1mM ammonium chloride and not that with 300mM ammonium chloride. The cell-free extracts from gdh^- mutant and prototroph were also examined with glutamine synthetase(GS) and glutamate synthase (GOGAT) production by niteogen sources. The growth of gdh^- mutant in presence of 20mM ammonium chloride means that GOGAT synthesis is sufficient to allow growth in this condition. GS production of gdh^- mutant as well as parental strain was induced by 1mM urea and ammonium tartrate, but it was repressed by higher concentration of ammonia, and also induced by 20mM to 50mM glutamate as a substrate. It was special attention that GOGAT synthesis from gdh^- strain was more repressed by higher concentration of ammonia than prototroph as described in E. coli system. | ||
| Download PDF | Kor_260204_93-100p.pdf | ||