| Title | 토양내에 고정화되어 있는 cellobiohydrolase의 효소학적 성질 | ||
| Author | 정종각 · 양영기 · 맹진수 · 이영하 | ||
| Address | 충남대학교 자연과학대학 미생물학과 | ||
| Bibliography | Korean Journal of Microbiology, 26(2),122-128, 1988 | ||
| DOI | |||
| Key Words | soil cellobiohydrolase, extracted cellobiohydrolase, immobilization | ||
| Abstract | The enzymatic properties of soil cellobiohydrolase were examined and compared with those of cellobiohydrolase-active extracts from soil in the forms of enzyme-humic complex and humicfree enzyme, and cellobiohydrolase partially pruified from Aspergillus niger. The pH optima of soil cellobiohydrolase and cellobiohydrolase-humic complex were greater by 1.5-3.0 pH units than those of cellobiohydrolase in humic-free extract and from A. niger. Soil cellobiohydrolase and cellobiohydrolase-humic complex were remarkably resistant to thermal denaturation and proteolysis. These results confirm that cellobiohydrolase in soil is stable in conditions which rapidly inactivate microbial cellobiohydrolase and that its stability is due to the immobilization of this enzyme by association with humic substances. The Michaelis-Menten constants (Km) for soil, cellobiohydrolase-humic complex, humic-free extract and cellobiohydrolase from A. niger were 22.1mg/ml, 11.3mg/ml, 10.6mg/ml and 4.5 mg/ml of Avicel, respectively. | ||
| Download PDF | Kor_260208_122-128p.pdf | ||