| Title | Rotaviruses의 염기배열 유사성 측정 | ||
| Author | 양재명 · Rex S. Spendlove * · Bill B. Barnett ** | ||
| Address | 경희대학교 자연과학대학 유전과학과; * Department og Biology, Utah State University | ||
| Bibliography | Korean Journal of Microbiology, 26(3),155-161, 1988 | ||
| DOI | |||
| Key Words | Rotavirus, Hybridization, RNA sequence homology | ||
| Abstract | Nucleotide sequence homology between bovine, simian, and porcine rotavirus was determined by the RNA:RNA hybridization technique. Single stranded RNA, prepared in vitro with EDTA activated endogeneous viral RNA polymerase, was hhbridized with tritium labeled bovine rotavirus genomic RNA. The heteroduplex RNA was treated with single stranded RNA specific ribonucleases and the RNase resistant hybrid RNA was precipitated, and collected by filtration on a filter paper. Seventy four percent RNA sequence homology between bovine and simian rotavirus and 8 percent RNA sequence homology between bovine and porcine rotavirus was confirmed by hybridization between tritium labeled single stranded RNA and viral genomic RNA. | ||
| Download PDF | Kor_260302_155-161p.pdf | ||