| Title | 원숭이 신장 세포에서 N13 DNA에 의한 SV40 DNA 복제 억제 현상에 대하여 | ||
| Author | 김연수 · 구용의 · 강현삼 | ||
| Address | 서울대학교 자연과학대학 미생물학과 | ||
| Bibliography | Korean Journal of Microbiology, 26(3),162-166, 1988 | ||
| DOI | |||
| Key Words | SV40 replication origin, M13 DNA, poison sequence | ||
| Abstract | Bacteriophage M13 DNAs carrying the wild type or base substituted SV40 DNA replication origins were used for replication assay. In vivo and in vitro assay with African green monkey cell line COS-1 showed that the replication of M13-SV40 recombinant DNAs was restricted like a pBR322 SV40 recombinant DNA(Lusky and Botchan, 1981). Furthermore, recombinant phage DNAs isolated from the transfected siminan cells subsequently show a reduced ability to retransform E. coli. But pATSV-W(Kim et al., 1988) was replicated in COS-1 cells normally. We think that a poison sequence may exist on bacteriophage M13 DNA like pBR322. | ||
| Download PDF | Kor_260303_162-166p.pdf | ||